Large-Scale In Vitro Propagation and Ex Vitro Adaptation of the Endangered Medicinal Plant Eryngium maritimum L.

The endangered medicinal plant Eryngium maritimum L. faces significant natural and anthropogenic threats. Therefore, in vitro propagation is recommended for both conservation commercial purposes. aim of the study was to develop a series protocols seed disinfection, multiplication rooting, ex field adaptation. For explant length temperature three consecutive disinfectants were investigated. Macrosalt modifications MS medium growth regulator addition media effect on axillary bud rate rooting studied. Survival leaf during adaptation response potting media, pot cell diameter, light spectrum tested. Seeds treated with diluted detergent at 40 °C 180 min, followed by 0.01% KMnO4 3 h bleach 11 achieved lower contamination high germination rate. Axillary proliferation enhanced reducing nitrogen content adding regulators. Potting size affect survival vitro. Timely transplantation conditions before overwintering increases plantlet survival. In present work, suitable foundation laid scaleup production E. micropropagation.